Thin-layer electrophoresis of serum lipoproteins

Thin-layer electrophoresis of serum lipoproteins

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A method is described for semiquantitative study of serum lipoproteins, with particular reference to the very low density fractions involved 8 GRAIN CEREAL in transport of triglycerides.Serum samples were subjected to electrophoresis in thin layers of starch granules; lipids were extracted from starch segments and subjected to thin-layer chromatography; lipid fractions were recovered from the adsorbent and quantified colorimetrically.The procedure is applicable to the study of alimentary and endogenous hyperlipidemias in Baby Powder man, and to radioactive tracer experiments.Over-all recoveries of lipids were variable and averaged 81% for cholesterol and 76% for triglycerides.